ALDH1A1 Antibody

AMM08753G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價(jià)格	詢價(jià)
AMM08753G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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ALDH1A1 Antibody

品牌	Leading Biology	貨號	AMM08753G
產(chǎn)品分類	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ALDH1A1 antibody.
分子量	H=55 KDa
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm.
宿主	Mouse
種屬反應(yīng)性	Human
免疫原	7-306 aa
靶點(diǎn)	This ALDH1A1 monoclonal antibody is generated from mouse immunized with ALDH1A1 recombinant protein.
通用名	ALDC, ALDH1, PUMB1
基因ID	216
UniProt ID	P00352
功能	Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid (By similarity).
總結(jié)	ALDH1A1 encodes a transcriptional regulator belonging to the SCY1-like family of kinase-like proteins. The protein has a divergent N-terminal kinase domain that is thought to be catalytically inactive, and can bind specific DNA sequences through its C-terminal domain. It activates transcription of the telomerase reverse transcriptase and DNA polymerase beta genes. The protein has been localized to the nucleus, and also to the cytoplasm and centrosomes during mitosis.
形式	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB
稀釋方法	FC~~1:25 WB~~1:1000
別名	Retinal dehydrogenase 1, RALDH 1, RalDH1, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Aldehyde dehydrogenase, cytosolic, ALDH1A1, ALDC, ALDH1, PUMB1
圖像	Overlay histogram showing A549 cells stained with AMM08753G(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM08753G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-ALDH1A1 Antibody at 1:1000 dilution Lane 1: HepG2 whole cell lysates Lane 2: K562 whole cell lysates Lane 3: NCI-H460 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Mouse IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
說明書	Product Datasheet Protocol
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