品牌 |
Leading Biology |
貨號 |
APR17127G |
產(chǎn)品分類 |
Monoclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality KRT5 antibody (monoclonal) (M08).
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分子量 |
62378 Da
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宿主 |
mouse
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種屬反應(yīng)性 |
Human
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靶點(diǎn) |
KRT5 (AAH24292, 1 a.a. ~ 590 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
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克隆號 |
1A12
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亞型 |
IgG2a Kappa
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基因ID |
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UniProt ID |
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總結(jié) |
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
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形式 |
Clear, colorless solution in phosphate buffered saline, pH 7.2 .
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IP, E
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稀釋方法 |
WB~~1:500~1000
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別名 |
Keratin, type II cytoskeletal 5, 58 kDa cytokeratin, Cytokeratin-5, CK-5, Keratin-5, K5, Type-II keratin Kb5, KRT5
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圖像 |
Western Blot analysis of KRT5 expression in transfected 293T cell line by KRT5 monoclonal antibody (M08), clone 1A12. Lane 1: KRT5 transfected lysate(62.4 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation of KRT5 transfected lysate using anti-KRT5 monoclonal antibody and Protein A Magnetic Bead (<a href="products_detail.asp?Catalog_id=U0007">U0007</a>), and immunoblotted with KRT5 MaxPab rabbit polyclonal antibody.
Detection limit for recombinant GST tagged KRT5 is 0.3 ng/ml as a capture antibody.
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說明書 |
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數(shù)量 |
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