PLIN3 Antibody

AMM07250G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
AMM07250G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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PLIN3 Antibody

品牌	Leading Biology	貨號	AMM07250G
產(chǎn)品分類	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PLIN3 antibody.
分子量	47075 Da
細胞定位	Antigen Cellular Localization: Cytoplasm. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lipid droplet. Note=Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails
宿主	Mouse
種屬反應(yīng)性	Human
靶點	This PLIN3 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-434 amino acids from human PLIN3.
克隆號	1651CT157.85.94
亞型	IgG2a,k
通用名	M6PRBP1, TIP47
基因ID	10226
UniProt ID	O60664
功能	Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.
總結(jié)	Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.
形式	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	FC, WB, E
稀釋方法	FC~~1:25 WB~~1:2000
別名	Perilipin-3, 47 kDa mannose 6-phosphate receptor-binding protein, 47 kDa MPR-binding protein, Cargo selection protein TIP47, Mannose-6-phosphate receptor-binding protein 1, Placental protein 17, PP17, PLIN3, M6PRBP1, TIP47
圖像	Overlay histogram showing HUVEC cells stained with AMM07250G(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM07250G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-PLIN3 Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Daudi whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST. All lanes : Anti-PLIN3 Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Daudi whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
說明書	Product Datasheet Protocol
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