KRT19 Antibody

APR17097G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR17097G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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KRT19 Antibody

品牌	Leading Biology	貨號	APR17097G
產(chǎn)品分類	Monoclonal Antibodies	研究領域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality KRT19 antibody.
分子量	44106 Da
宿主	Mouse
種屬反應性	Human
靶點	This antibody is generated from a mouse immunized with a recombinant protein of human.
克隆號	979CT14.3.1
亞型	IgG1,k
基因ID	3880
UniProt ID	P08727
功能	Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
總結	Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
形式	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應用	IF, IHC-P, WB, E
稀釋方法	IF~~1:25 IHC-P~~1:25 WB~~1:4000
別名	Keratin, type I cytoskeletal 19, Cytokeratin-19, CK-19, Keratin-19, K19, KRT19
圖像	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Pdx1 with APR17097G at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing intermediate filaments staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). APR17097G staining KRT19 in human kidney sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-KRT19 Antibody at 1:4000 dilution Lane 1: MCF-7 whole cell lysates Lane 2: HepG2 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
說明書	Product Datasheet Protocol
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