CAPN2 Antibody

APG02359G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢(xún)價(jià)
APG02359G		100 μl / ￥4950

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CAPN2 Antibody

品牌	Leading Biology	貨號(hào)	APG02359G
產(chǎn)品分類(lèi)	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CAPN2 antibody.
分子量	79995 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding
宿主	Mouse
種屬反應(yīng)性	Human
免疫原	1-400 aa
靶點(diǎn)	This CAPN2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human CAPN2.
克隆號(hào)	1381CT669.7.66.71
亞型	IgG2b
通用名	CANPL2
基因ID	824
UniProt ID	P17655
功能	Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508).
總結(jié)	Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
形式	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, FC, IHC-P, E
稀釋方法	IHC-P~~1:25 FC~~1:100 WB~~1:1000
別名	Calpain-2 catalytic subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, M-calpain, CAPN2, CANPL2
圖像	Immunohistochemical analysis of paraffin-embedded H.stomach section using CAPN2 Antibody( APG02359G). APG02359G was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining. Flow cytometric analysis of U-87 MG cells using CAPN2 Antibody(green, APG02359G) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor? 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody. Western blot analysis of lysates from A549, A431, SK-BR-3 cell line (from left to right), using CAPN2 Antibody( APG02359G). APG02359G was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
說(shuō)明書(shū)	Product Datasheet Protocol
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