ALDOC Antibody (C-term)

APG01805G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APG01805G		100 μl / ￥4950

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品牌	Leading Biology	貨號	APG01805G
產(chǎn)品分類	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ALDOC antibody (C-term).
分子量	39456 Da
宿主	Mouse
種屬反應(yīng)性	Human, Mouse, Rat
靶點	Purified His-tagged ALDOC protein was used to produced this monoclonal antibody.
克隆號	859CT9.5.3
亞型	IgG3
通用名	ALDC
基因ID	230
UniProt ID	P09972
形式	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	FC, WB, E
稀釋方法	FC~~1:25 WB~~1:1000
別名	Fructose-bisphosphate aldolase C, Brain-type aldolase, ALDOC, ALDC
圖像	Overlay histogram showing HL-60 cells stained with AM2215B(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM2215B, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG3 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-ALDOC Antibody (C-term) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: 293T/17 whole cell lysate Lane 3: mouse brain lysate Lane 4: rat brain lysate Lane 5: mouse spleen lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST. ALDOC Antibody (C-term)(Cat. APG01805G) western blot analysis in HL-60,293 cell line and mouse brain,spleen lysates (35μg/lane).This demonstrates the ALDOC antibody detected the ALDOC protein (arrow).
說明書	Product Datasheet Protocol
數(shù)量

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