品牌 |
Leading Biology | 貨號(hào) |
APR11292G |
產(chǎn)品分類 |
Monoclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality TBB5 antibody.
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分子量 |
49671 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm, cytoskeleton.
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宿主 |
Mouse
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種屬反應(yīng)性 |
Human, Mouse, Rat
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靶點(diǎn) |
TBB5 recombinant protein is used to produce this monoclonal antibody.
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克隆號(hào) |
87CT59.3.7
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亞型 |
IgG2b,Igκ
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通用名 |
TUBB5
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基因ID |
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UniProt ID |
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功能 |
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
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形式 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
IHC-P, WB, IF, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
IF~~1:25
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別名 |
Tubulin beta chain, Tubulin beta-5 chain, TUBB, TUBB5
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圖像 |
Western blot analysis of lysates from 293, CEM, Hela, Y79, rat PC-12 cell line, mouse brain tissue lysate(from left to right), using TBB5 Antibody( APR11292G). APR11292G was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20μg per lane. Immunohistochemical analysis of paraffin-embedded H.kidney section using TBB5 Antibody( APR11292G). APR11292G was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining. Immunofluorescent analysis of A549 cells, using TBB5 Antibody (Cat. APR11292G). APR11292G was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor? 554 (red) conjugated Phalloidin (red). |
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說(shuō)明書(shū) |
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數(shù)量 |
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