FASN Antibody (Center)

AMM04513G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
AMM04513G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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FASN Antibody (Center)

品牌	Leading Biology	貨號(hào)	AMM04513G
產(chǎn)品分類(lèi)	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality FASN antibody (Center).
分子量	273427 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
宿主	Mouse
種屬反應(yīng)性	Human, Mouse
免疫原	942-973 aa
靶點(diǎn)	This FASN antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 942-973 amino acids from the Central region of human FASN.
克隆號(hào)	497CT15.2.5
亞型	IgM
通用名	FAS
基因ID	2194
UniProt ID	P49327
功能	Fatty acid synthetase catalyzes the formation of long- chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.
總結(jié)	The enzyme encoded by this gene is a multifunctionalprotein. Its main function is to catalyze the synthesis ofpalmitate from acetyl-CoA and malonyl-CoA, in the presence ofNADPH, into long-chain saturated fatty acids. In some cancer celllines, this protein has been found to be fused with estrogenreceptor-alpha (ER-alpha), in which the N-terminus of FAS is fusedin-frame with the C-terminus of ER-alpha.
形式	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IF, E
稀釋方法	WB~~1:100 IF~~1:200
別名	Fatty acid synthase, [Acyl-carrier-protein] S-acetyltransferase, [Acyl-carrier-protein] S-malonyltransferase, 3-oxoacyl-[acyl-carrier-protein] synthase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, Enoyl-[acyl-carrier-protein] reductase, Oleoyl-[acyl-carrier-protein] hydrolase, FASN, FAS
圖像	Anti- at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 273 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Fluorescent confocal image of HepG2 cells stained with FASN (Center) antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AMM04513G FASN primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the FASN immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000169710). FASN Antibody (Center) (Cat. AMM04513G) western blot analysis in mouse brain tissue lysates (35μg/lane).This demonstrates the FASN (Center) antibody detected the FASN (Center) protein (arrow).
說(shuō)明書(shū)	Product Datasheet Protocol
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