品牌 |
Leading Biology | 貨號(hào) |
APR10255G |
產(chǎn)品分類 |
Monoclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Stat-5 alpha antibody (C-term).
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分子量 |
90647 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Rat
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靶點(diǎn) |
A synthetic peptide corresponding to residues in C-terminus of human Stat5a was used as immunogen. The antibody does not cross-react with other Stat family members.
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克隆號(hào) |
E289
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通用名 |
STAT5
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基因ID |
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UniProt ID |
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功能 |
Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
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總結(jié) |
Signal transducer and activator of transcription 5 (Stat5) functions both in signal transduction and activation of transcription. It has been found that cytoplasmic Stat5 is translocated to the nucleus in response to phosphorylation. Stat5 is tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin (1,2). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. NCoA-1/SRC-1 acts as a coactivator for both the a- and b-isoforms of Stat5 (3).
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形式 |
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC
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稀釋方法 |
WB~~1:1000~10000
IHC~~1:100~250
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別名 |
Signal transducer and activator of transcription 5A, STAT5A, STAT5
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圖像 |
A. Western blot analysis on A431 cell lysate using anti- Stat5a RabMAb (Cat. APR10255G), dilution 1:1,000. B. Immunohistochemical analysis of paraffin-embedded human squamous lung carcinoma using anti-STAT5a RabMAb (Cat. APR10255G). |
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說明書 |
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數(shù)量 |
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