品牌 |
Leading Biology | 貨號(hào) |
AMM06651G |
產(chǎn)品分類 |
Monoclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Neurofilament-66 antibody.
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分子量 |
55391 Da
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Rat
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靶點(diǎn) |
A synthetic peptide corresponding to residues in the tail domain of human NF-66 was used as immunogen.
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克隆號(hào) |
EP676Y
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通用名 |
NEF5
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基因ID |
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UniProt ID |
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功能 |
Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
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總結(jié) |
Neurofilaments (NF) are cytoplasmic intermediate filaments (IFs) found in neurons. NF-66, also known as α-internexin, is a member of the IF family. Classified as a type IV IF, NF-66 is expressed by most neurons and has been shown to co-assemble with other NF triple proteins (light, medium and heavy NF) (1-2). In the adult brain, NF-66 is expressed at relatively low levels compared to the other NF proteins (3). NF-66 has also been shown to assemble into 10mm diameter IF in vitro.
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形式 |
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IF, IHC
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稀釋方法 |
WB~~1:1000~5000
IHC~~1:250~500
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別名 |
Alpha-internexin, Alpha-Inx, 66 kDa neurofilament protein, NF-66, Neurofilament-66, Neurofilament 5, INA, NEF5
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圖像 |
A. Western blot analysis on SHSY5Y cell lysate using anti-NF-66 RabMAb (Cat. AMM06651G). B. Immunofluorescent staining of PC 12 cells using anti-NF-66 RabMAb (Cat. AMM06651G) C. Positive immunohistochemical staining of paraffin-embedded human brain tissue (A.) using anti-NF-66 RabMAb. |
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說明書 |
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數(shù)量 |
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