品牌 |
Leading Biology |
貨號(hào) |
APR08657G |
產(chǎn)品分類 |
Monoclonal Antibodies |
研究領(lǐng)域 |
|
產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality NDUFAF1 antibody.
|
分子量 |
37764 Da
|
細(xì)胞定位 |
Antigen Cellular Localization:
Mitochondrion.
|
宿主 |
Rabbit
|
種屬反應(yīng)性 |
Human, Mouse, Rat
|
靶點(diǎn) |
A synthetic peptide corresponding to residues at the C terminus of human NDUFAF1 was used as an immunogen.
|
克隆號(hào) |
EPR2796
|
通用名 |
CIA30
|
基因ID |
|
UniProt ID |
|
功能 |
Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).
|
總結(jié) |
NDUFAF1 (CIA30) is a chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex 1) (1). Complex I is the largest multiprotein enzyme located in the inner mitochondrial membrane that catalyzes the transfer of electrons from NADH to coenzyme Q (CoQ). Little is known about the assembly process of the subunits into the mature complex, however, NDUFAF1 is one of two proteins found to be involved. NDUFAF1 expression is ubiquitous with a slightly higher expression in various heart tissues, kidney, lung and liver (2).
|
形式 |
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
|
儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
|
應(yīng)用 |
WB, IHC, IF
|
稀釋方法 |
WB~~1:1000~2000
IHC~~1:100~250
|
別名 |
Complex I intermediate-associated protein 30, mitochondrial, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1, NDUFAF1, CIA30
|
圖像 |
A. Western blot analysis on (A) HeLa, (B) Jurkat and (C) human heart lysates using anti-NDUFAF1 RabMAb (Cat. APR08657G).
B. Immunohistochemical analysis of paraffin-embedded human liver using anti-NDUFAF1 RabMAb (Cat. APR08657G).
C. Immunofluorescent staining of HeLa cells using anti-NDUFAF1 RabMAb (Cat. APR08657G).
|
說明書 |
|
數(shù)量 |
|