ILF3 Antibody

APR16834G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR16834G		100 μl / ￥5450

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安諾倫（北京）生物科技有限公司

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ILF3 Antibody

品牌	Leading Biology	貨號(hào)	APR16834G
產(chǎn)品分類	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ILF3 antibody.
分子量	95338 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus, nucleolus. Cytoplasm. Nucleus. Note=Localizes in the cytoplasm in response to viral dsRNA. The unphosphorylated form is retained in the nucleus by ILF2 Phosphorylation at Thr-188 and Thr-315 causes the dissociation of ILF2 from the ILF2-ILF3 complex resulting in a cytoplasmic sequestration of ILF3. Localized in cytoplasmic mRNP granules containing untranslated mRNAs
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
靶點(diǎn)	A synthetic peptide corresponding to residues in human ILF3 was used as an immunogen.
克隆號(hào)	EPR3626
通用名	DRBF, MPHOSPH4, NF90
基因ID	3609
UniProt ID	Q12906
功能	May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N- methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. The phosphorylated form at Thr-188 and Thr-315, in concert with EIF2AK2/PKR can inhibit vesicular stomatitis virus (VSV) replication (By similarity).
總結(jié)	Interleukin enhancer-binding factor 3 (ILF3) is a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein was first discovered to be a subunit of the nuclear factor of activated T-cells (NFAT); a transcription factor required for T-cell expression of interleukin 2 (1). ILF3 regulates protein arginine N-methyltransferase 1 activity and has been suggested to regulate transcription of the IL2 gene during T-cell activation. ILF3 promotes the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA (2).
形式	50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC
稀釋方法	WB~~1:5000~10000 IHC~~1:100~250
別名	Interleukin enhancer-binding factor 3, Double-stranded RNA-binding protein 76, DRBP76, M-phase phosphoprotein 4, MPP4, Nuclear factor associated with dsRNA, NFAR, Nuclear factor of activated T-cells 90 kDa, NF-AT-90, Translational control protein 80, TCP80, ILF3, DRBF, MPHOSPH4, NF90
圖像	Western blot analysis on (A) Raji, (B) HeLa and (C) K562 cell lysates using anti-ILF3 RabMAb (Cat. APR16834G), dilution 1:100,000. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ILF3 RabMAb (Cat. APR16834G).
說明書	Product Datasheet Protocol
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