CAD Antibody

APG02330G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APG02330G		100 μl / ￥5450

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安諾倫（北京）生物科技有限公司

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CAD Antibody

品牌	Leading Biology	貨號(hào)	APG02330G
產(chǎn)品分類	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CAD antibody.
分子量	242984 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm. Nucleus. Note=Cytosolic and unphosphorylated in resting cells, translocates to the nucleus in response to EGF stimulation, nuclear import promotes optimal cell growth
宿主	Rabbit
種屬反應(yīng)性	Human
靶點(diǎn)	A synthetic peptide corresponding to residues near the C-terminus of humanCAD was used as immunogen.
克隆號(hào)	EP711Y
基因ID	790
UniProt ID	P27708
功能	This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).
總結(jié)	Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis (1). This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway (2). Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus (3). While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD (4-5).
形式	50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC
稀釋方法	WB~~1:1000~10000 IHC~~1:100~250
別名	CAD protein, Glutamine-dependent carbamoyl-phosphate synthase, Aspartate carbamoyltransferase, Dihydroorotase, CAD
圖像	A. Western blot analysis on 293 cell lysate using anti-CAD RabMAb (Cat. APG02330G). B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-CAD RabMAb (Cat. APG02330G).
說明書	Product Datasheet Protocol
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