4E-BP1 Antibody

APG01380G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢(xún)價(jià)
APG01380G		100 μl / ￥5450

大規(guī)格詢(xún)價(jià)

2 Images

訂購(gòu)信息

安諾倫（北京）生物科技有限公司

Tel: 400-965-8633
Tel: 010-56256916
Inquiry@annoron.com

全國(guó)授權(quán)代理商

4E-BP1 Antibody

品牌	Leading Biology	貨號(hào)	APG01380G
產(chǎn)品分類(lèi)	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality 4E-BP1 antibody.
分子量	12580 Da
宿主	Rabbit
種屬反應(yīng)性	Human
靶點(diǎn)	A synthetic peptide corresponding to residues near the C-term of human4E-BP1 was used as immunogen.
克隆號(hào)	Y330
基因ID	1978
UniProt ID	Q13541
功能	Regulates eIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
總結(jié)	4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will not (2). Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4)
形式	50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC
稀釋方法	WB~~1:1000 IHC~~1:50~100
別名	Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, EIF4EBP1
圖像	A. Western blot analysis on K562 cell lysate using anti-4E-BP1 RabMAb (Cat. APG01380G), dilution 1:1,000. B. Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using anti-4E-BP1 RabMAb (Cat. APG01380G).
說(shuō)明書(shū)	Product Datasheet Protocol
數(shù)量

加入購(gòu)物車(chē) 詢(xún)價(jià)

上一產(chǎn)品：4E-BP1 Antibody Phospho (pT37) 下一產(chǎn)品：CD20 / MS4A1 (B-Cell Marker) Antibody - Culture Supernatant

熱銷(xiāo)產(chǎn)品

相關(guān)產(chǎn)品

選擇	品牌	貨號(hào)	產(chǎn)品名稱(chēng)	規(guī)格	分類(lèi)	研究領(lǐng)域	說(shuō)明書(shū)	數(shù)量	目錄價(jià)
1	Leading Biology	APG01369G	VEGF (Vascular Endothelial Growth Factor) Antibody - Without BSA and Azide	100 μg	Monoclonal Antibodies				￥4950.00	訂購(gòu) 詢(xún)價(jià)
2	Leading Biology	AMM07627G	RIN2 Antibody (monoclonal) (M01)	100 μg	Monoclonal Antibodies				￥4950.00	訂購(gòu) 詢(xún)價(jià)
3	Leading Biology	APR15980G	FGG Antibody	100 μl	Monoclonal Antibodies				￥5450.00	訂購(gòu) 詢(xún)價(jià)
4	Leading Biology	AMM05161G	GOB5 / CLCA1 Antibody (clone 1C4)	50 μg	Monoclonal Antibodies				￥5450.00	訂購(gòu) 詢(xún)價(jià)
5	Leading Biology	APR17667G	ORAI1 Antibody [3F6H5]	0.1 mg	Monoclonal Antibodies				￥6950.00	訂購(gòu) 詢(xún)價(jià)
6	Leading Biology	APR11118G	HIV-1 p24 Antibody [7F4] (biotin)	0.1 mg	Monoclonal Antibodies				￥6950.00	訂購(gòu) 詢(xún)價(jià)