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MuRF1 Antibody

AMM03014G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
AMM03014G		100 μl / ￥5450

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MuRF1 Antibody

品牌	Leading Biology	貨號	AMM03014G
產(chǎn)品分類	Monoclonal Antibodies	研究領域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality MuRF1 Antibody.
分子量	40.2kDa
細胞定位	Antigen Cellular Localization: Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, M line. Cytoplasm, myofibril, sarcomere, Z line. Note=Colocalizes with TNNI3 in myocytes (By similarity) Localizes to the M- and Z-lines in skeletal muscle.
宿主	Mouse
種屬反應性	Human
克隆號	7B8E1
亞型	IgG1
通用名	IRF, MURF1, RNF28, SMRZ
基因ID	84676
UniProt ID	Q969Q1
功能	E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti- hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應用	IHC, FC, ICC, E
稀釋方法	E~~1/10000 IF~~1/200 - 1/1000 FC~~1/200 - 1/400 IHC~~1/200 - 1/1000
圖像	Black line: Control Antigen (100 ng);Purple line: Antigen(10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng); Figure 1:Immunofluorescence analysis of Hela cells using MuRF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher ( : 35503) Figure 2: Flow cytometric analysis of Hela cells using MuRF1 mouse mAb (green) and negative control (red).
說明書	Product Datasheet Protocol
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