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當(dāng)前位置：首頁(yè) > 抗原抗體、ELISA、WB > 一抗 > Monoclonal Antibodies > NEDD8 Antibody (clone 5B8)

NEDD8 Antibody (clone 5B8)

貨號(hào) 貨期 規(guī)格 / 價(jià)格 詢價(jià)
AMM02245G 50 μg / ￥4950

NEDD8 Antibody (clone 5B8)

品牌

 Leading Biology

貨號(hào)

 AMM02245G

產(chǎn)品分類

 Monoclonal Antibodies

研究領(lǐng)域

產(chǎn)品概述
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality NEDD8 Antibody (clone 5B8).

分子量
9kDa

細(xì)胞定位
Antigen Cellular Localization: Nucleus. Note=Mainly nuclear

宿主
Mouse

種屬反應(yīng)性
Human

靶點(diǎn)
Human NEDD8

克隆號(hào)
5B8

基因ID

UniProt ID

功能
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

總結(jié)
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

儲(chǔ)存條件
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

應(yīng)用
WB, IHC-P, IF, ICC, E, Flo

稀釋方法
ELISA (1:10000), Flo (1:200-1:400), ICC (1:200-1:1000), IF (1:1000), IHC-P (10 μg/ml), WB (1:500-1:2000),

圖像

Anti-NEDD8 antibody IHC staining of human brain, cerebellum.

Western blot using NEDD8 mouse monoclonal antibody against C6 (1) and HeLa (2) cell lysate.

Immunofluorescence of HeLa cells using NEDD8 mouse monoclonal antibody (green).

說(shuō)明書(shū)

數(shù)量

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