USP14 Antibody (N-term)

AMM04353G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
AMM04353G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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USP14 Antibody (N-term)

品牌	Leading Biology	貨號	AMM04353G
產(chǎn)品分類	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality USP14 Antibody (N-term).
分子量	H=56;M=56;R=56 KDa
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm. Cell membrane; Peripheral membrane protein
宿主	Mouse
種屬反應(yīng)性	Human, Mouse
免疫原	1-494 aa
靶點	Purified His-tagged USP14 protein was used to produced this monoclonal antibody.
通用名	TGT
基因ID	9097
UniProt ID	P54578
功能	Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs).
總結(jié)	Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs).
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB
稀釋方法	FC~~1:25 WB~~1:2000
圖像	Overlay histogram showing Jurkat cells stained with AMM04353G(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM04353G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-USP14 Antibody (N-term) at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: mouse brain whole cell lysate Lane 3: Jurkat whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
說明書	Product Datasheet Protocol
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