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當(dāng)前位置：首頁(yè) > 抗原抗體、ELISA、WB > 一抗 > Monoclonal Antibodies > SETDB1 Antibody

SETDB1 Antibody

貨號(hào) 貨期 規(guī)格 / 價(jià)格 詢(xún)價(jià)
AMM02807G 100 μl / ￥4950

SETDB1 Antibody

品牌

 Leading Biology

貨號(hào)

 AMM02807G

產(chǎn)品分類(lèi)

 Monoclonal Antibodies

研究領(lǐng)域

產(chǎn)品概述
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SETDB1 Antibody.

分子量
180kDa

細(xì)胞定位
Antigen Cellular Localization: Nucleus. Chromosome. Note=Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin

宿主
Mouse

種屬反應(yīng)性
Human, Mouse, Monkey

克隆號(hào)
5H6A12

亞型
IgG1

通用名
KIAA0067, KMT1E

基因ID

UniProt ID

功能
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.

儲(chǔ)存條件
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

應(yīng)用
WB, ICC, E

稀釋方法
WB~~1/500 - 1/2000 IF~~1/200 - 1/1000

圖像

Figure 1: Western blot analysis using SETDB1 mouse mAb against MCF-7 (1),T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5), F9 (6), RAW246.7 (7) and Cos7 (8) cell lysate.

Figure 2: Immunofluorescence analysis of LOVO cells using SETDB1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

說(shuō)明書(shū)

數(shù)量

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