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當(dāng)前位置：首頁(yè) > 抗原抗體、ELISA、WB > 一抗 > Monoclonal Antibodies > RICTOR Antibody

RICTOR Antibody

貨號(hào) 貨期 規(guī)格 / 價(jià)格 詢價(jià)
AMM02786G 100 μl / ￥4950

RICTOR Antibody

品牌

 Leading Biology

貨號(hào)

 AMM02786G

產(chǎn)品分類

 Monoclonal Antibodies

研究領(lǐng)域

產(chǎn)品概述
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RICTOR Antibody.

分子量
192kDa

宿主
Mouse

種屬反應(yīng)性
Human, Mouse, Monkey

克隆號(hào)
7B3

亞型
IgG1

基因ID

UniProt ID

功能
Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient- insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.

儲(chǔ)存條件
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

應(yīng)用
WB, IHC, FC, ICC, E

稀釋方法
WB~~1/500 - 1/2000 IF~~1/200 - 1/1000 IHC~~1/500 - 1/2000 FC~~1/200 - 1/400

圖像

Figure 1: Western blot analysis using RICTOR mouse mAb against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysate.

Figure 2: Immunofluorescence analysis of NIH/3T3 cells using RICTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Figure 3: Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and human breast carcinoma (right) using RICTOR mouse mAb with DAB staining.

說(shuō)明書(shū)

數(shù)量

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