品牌 |
Leading Biology |
貨號 |
AMM02707G |
產(chǎn)品分類 |
Monoclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality BRAF Antibody.
|
分子量 |
87kDa
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細胞定位 |
Antigen Cellular Localization:
Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
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宿主 |
Mouse
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種屬反應(yīng)性 |
Human, Mouse
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克隆號 |
1H12
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亞型 |
IgG1
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通用名 |
BRAF1, RAFB1
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基因ID |
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UniProt ID |
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功能 |
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC, ICC, E
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稀釋方法 |
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
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圖像 |
Figure 1: Western blot analysis using BRAF mouse mAb against Hela (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
Figure 2: Immunohistochemical analysis of paraffin-embedded human testis tissues using BRAF mouse mAb.
Figure 3: Confocal immunofluorescence analysis of MCF-7 (left) and HepG2 (right) cells using BRAF mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye
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說明書 |
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數(shù)量 |
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