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Nestin Antibody

貨號(hào) 貨期 規(guī)格 / 價(jià)格 詢(xún)價(jià)
AMM02595G 100 μl / ¥4950

Nestin Antibody

品牌

Leading Biology

貨號(hào)

AMM02595G

產(chǎn)品分類(lèi)

Monoclonal Antibodies

研究領(lǐng)域

產(chǎn)品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Nestin Antibody.

分子量

240 KDa

宿主

Mouse

種屬反應(yīng)性

Human, Mouse, Rat

靶點(diǎn)

Recombinant human Nestin expressed in and purifeid from E. coli.

克隆號(hào)

4D11

亞型

IgG1

基因ID

UniProt ID

總結(jié)

Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells (Lendahl et al., 1990). Nestin was originally identified as a result of the production of a series of monoclonal antibodies directed against epitopes expressed on formalin fixed embryo day 15 rat spinal cord (Hockfield et al., 1985)). One of these antibodies, called Rat 401, stained fibrous profiles in the developing nervous system, but not in the mature nervous system. By screening bacteriophage expression libraries with Rat 401, Lendahl et al. were able to isolate a cDNA encoding the protein to which Rat 401 antibody bound. The protein proved to be an unusual member of the intermediate filament family, containing an alpha-helical region homologous to that found in keratin and neurofilament subunits.

儲(chǔ)存條件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

應(yīng)用

WB, IF

稀釋方法

WB~~ 1:2000 IF~~ 1:500

圖像

Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~220-240 k nestin doublet.

Immunochemical staining of cultured neonatal rat neurons and glia showing nestin labeling in red and vimentin in green. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.

說(shuō)明書(shū)

數(shù)量

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