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> 抗原抗體、ELISA、WB > 一抗 > Monoclonal Antibodies > Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) Antibody品牌 |
Leading Biology | 貨號 |
AMM02589G |
產(chǎn)品分類 |
Monoclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) Antibody.
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分子量 |
38 KDa
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宿主 |
mouse
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種屬反應(yīng)性 |
Human, Mouse, Rat
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靶點 |
Purified porcine GAPDH.
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克隆號 |
1D4
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亞型 |
IgM
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基因ID |
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UniProt ID |
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總結(jié) |
Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription (1). GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease (2,3). Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic (4).
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IF
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稀釋方法 |
WB~~ 1:1000
IF~~ 1:100
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圖像 |
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說明書 |
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數(shù)量 |
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