RAB3A Antibody

AMM02525G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
AMM02525G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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RAB3A Antibody

品牌	Leading Biology	貨號(hào)	AMM02525G
產(chǎn)品分類	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RAB3A Antibody.
分子量	24984 Da
細(xì)胞定位	Antigen Cellular Localization: Cell membrane; Lipid-anchor; Cytoplasmic side
宿主	Mouse
種屬反應(yīng)性	Human, Mouse, Rat
靶點(diǎn)	This RAB3A antibody is generated from a mouse immunized with a recombinant protein.
克隆號(hào)	1531CT562.14.57
亞型	IgG1,k
基因ID	5864
UniProt ID	P20336
功能	Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.
總結(jié)	Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	IF, FC, WB, E
稀釋方法	IF~~1:25 FC~~1:25 WB~~1:500
圖像	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized PC-12 (rat adrenal phaeochromocytoma cell line) cells labeling Pdx1 with AMM02525G at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on PC-12 cell line. Cytoplasmic actin is detected with Dylight? 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue). Overlay histogram showing PC-12 cells stained with AMM02525G (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM02525G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-RAB3A Antibody at 1:2000 dilution Lane 1: mouse brain lysate Lane 2: rat brain lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
說(shuō)明書	Product Datasheet Protocol
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