TOP1 Antibody (N-term)

AMM02480G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢(xún)價(jià)
AMM02480G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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TOP1 Antibody (N-term)

品牌	Leading Biology	貨號(hào)	AMM02480G
產(chǎn)品分類(lèi)	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality TOP1 Antibody (N-term).
分子量	90726 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli
宿主	Mouse
種屬反應(yīng)性	Human
靶點(diǎn)	This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1.
克隆號(hào)	1291CT875.142.166
亞型	IgG1,κ
基因ID	7150
UniProt ID	P11387
功能	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.
總結(jié)	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, FC, E
稀釋方法	FC~~1:25 WB~~1:1000
圖像	Flow cytometric analysis of Hela cells using TOP1 Antibody (N-term)(green, AMM02480G) compared to an isotype control of mouse IgG1(blue). AMM02480G was diluted at 1:25 dilution. An Alexa Fluor? 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody. Western blot analysis of lysates from HUVEC, Jurkat, MCF-7, PC-12 cell line (from left to right), using TOP1 Antibody (N-term) (Cat. AMM02480G). AMM02480G was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
說(shuō)明書(shū)	Product Datasheet Protocol
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