ERCC1 Antibody (C-term)

AMM02449G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
AMM02449G		100 μl / ￥4950

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品牌	Leading Biology	貨號	AMM02449G
產(chǎn)品分類	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ERCC1 Antibody (C-term).
分子量	32562 Da
細(xì)胞定位	Antigen Cellular Localization: Isoform 1: Nucleus. Isoform 3: Nucleus.
宿主	Mouse
種屬反應(yīng)性	Human
免疫原	268-297 aa
靶點(diǎn)	This ERCC1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 268-297 amino acids from the C-terminal region of human ERCC1.
克隆號	752CT13.2.5
亞型	IgM,k
基因ID	2067
UniProt ID	P07992
功能	Isoform 1: Non-catalytic component of a structure- specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.
總結(jié)	Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, E
稀釋方法	WB~~1:1000
圖像	All lanes : Anti-ERCC1 Antibody (C-term) at 1:2000 dilution Lane 1: A549 whole cell lysates Lane 2: Hela whole cell lysates Lane 3: T47D whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of lysates from A549, T47D cell line (from left to right), using ERCC1 Antibody (C-term)(AMM02449G). AMM02449G was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 20μg per lane. ERCC1 Antibody (C-term) (Cat. AMM02449G) western blot analysis in Hela,A549 cell line lysates (35μg/lane).This demonstrates the ERCC1 antibody detected the ERCC1 protein (arrow).
說明書	Product Datasheet Protocol
數(shù)量

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