KIT Antibody

AMM02436G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
AMM02436G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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KIT Antibody

品牌	Leading Biology	貨號(hào)	AMM02436G
產(chǎn)品分類	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality KIT Antibody .
分子量	109865 Da
細(xì)胞定位	Antigen Cellular Localization: Isoform 1: Cell membrane; Single-pass type I membrane protein Isoform 3: Cytoplasm. Note=Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head
宿主	Mouse
種屬反應(yīng)性	Human
靶點(diǎn)	This KIT Antibody is generated from mouses immunized with human KIT recombinant protein.
克隆號(hào)	566CT8.5.4
亞型	IgM
通用名	SCFR
基因ID	3815
UniProt ID	P10721
功能	Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
總結(jié)	This gene encodes the human homolog of the proto-oncogenec-kit. C-kit was first identified as the cellular homolog of thefeline sarcoma viral oncogene v-kit. This protein is a type 3transmembrane receptor for MGF (mast cell growth factor, also knownas stem cell factor). Mutations in this gene are associated withgastrointestinal stromal tumors, mast cell disease, acutemyelogenous lukemia, and piebaldism. Multiple transcript variantsencoding different isoforms have been found for this gene.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IF, E
稀釋方法	IF~~1:10~50 WB~~1:100
圖像	Immunofluorescent analysis of U251 cells, using KIT Antibody (Cat. AMM02436G). AMM02436G was diluted at 1:100 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor? 554 (red) conjugated Phalloidin (red). KIT Antibody(Cat. AMM02436G) western blot analysis in A549 cell line lysates (35μg/lane).This demonstrates the KIT antibody detected the KIT protein (arrow). Fluorescent confocal image of an SY5Y (SH-SY5Y) cell stained with AMM02436G KIT antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AMM02436G KIT primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 3 min). Note the highly specific localization of the KIT immunosignal to the membranes, especially the plasma membrane. ibid. References for SY5Y (SH-SY5Y; ATCCCRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704]. Ibid.
說(shuō)明書	Product Datasheet Protocol
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