品牌 |
Leading Biology |
貨號 |
AMM02407G |
產(chǎn)品分類 |
Monoclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PSMA5 Antibody.
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分子量 |
26411 Da
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細胞定位 |
Antigen Cellular Localization:
Cytoplasm. Nucleus.
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宿主 |
Mouse
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種屬反應(yīng)性 |
Human, Mouse, Rat
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靶點 |
Purified His-tagged PSMA5 protein(Fragment) was used to produced this monoclonal antibody.
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克隆號 |
426CT8.5.1
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亞型 |
IgG1
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基因ID |
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UniProt ID |
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功能 |
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
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總結(jié) |
The proteasome is a multicatalytic proteinase complex witha highly ordered ring-shaped 20S core structure. The core structureis composed of 4 rings of 28 non-identical subunits; 2 rings arecomposed of 7 alpha subunits and 2 rings are composed of 7 betasubunits. Proteasomes are distributed throughout eukaryotic cellsat a high concentration and cleave peptides in anATP/ubiquitin-dependent process in a non-lysosomal pathway. Anessential function of a modified proteasome, the immunoproteasome,is the processing of class I MHC peptides. This gene encodes amember of the peptidase T1A family, that is a 20S core alphasubunit.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, IF, E
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稀釋方法 |
WB~~1:100~500
IF~~1:25
IHC-P~~1:25
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圖像 |
All lanes : Anti-PSMA5 Antibody at 1:1000 dilution
Lane 1: human liver lysate
Lane 2: Hela whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: Neuro-2a whole cell lysate
Lane 6: PC-12 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 26 kDa.
Blocking/Dilution buffer: 5% NFDM/TBST.
Fluorescent image of Hela cells stained with XAF1 PSMA5 Antibody( AMM02407G). AMM02407G was diluted at 1:25 dilution. An Alexa Fluor? 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded H. testis section using PSMA5 Antibody( AMM02407G). AMM02407G was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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說明書 |
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數(shù)量 |
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