品牌 |
Leading Biology |
貨號 |
AMM02446G |
產(chǎn)品分類 |
Monoclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality SPARC Antibody(C-term) Antibody(Ascites).
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分子量 |
34632 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Secreted, extracellular space, extracellular matrix, basement membrane. Note=In or around the basement membrane
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宿主 |
Mouse
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種屬反應(yīng)性 |
Human
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免疫原 |
224-251 aa
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靶點(diǎn) |
This SPARC antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human SPARC.
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克隆號 |
664CT7.3.1
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亞型 |
IgG1
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通用名 |
ON
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基因ID |
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UniProt ID |
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功能 |
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
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總結(jié) |
Secreted protein acidic and rich incysteine/osteonectin/BM40, or SPARC, is a matrix-associated proteinthat elicits changes in cell shape, inhibits cell-cycleprogression, and influences the synthesis of extracellular matrix(ECM) (Bradshaw et al., 2003 [PubMed 12721366]).
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, E
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稀釋方法 |
WB~~1:100~800
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圖像 |
SPARC Antibody (C-term)(Ascites)(Cat. AMM02446G) western blot analysis in Hela cell line lysates (35μg/lane).This demonstrates the SPARC antibody detected the SPARC protein (arrow).
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說明書 |
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數(shù)量 |
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