PODXL Antibody (C-term) (Ascites)

AMM02439G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢(xún)價(jià)
AMM02439G		0.1 ml / ￥4950

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PODXL Antibody (C-term) (Ascites)

品牌	Leading Biology	貨號(hào)	AMM02439G
產(chǎn)品分類(lèi)	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PODXL Antibody (C-term) (Ascites).
分子量	58635 Da
細(xì)胞定位	Antigen Cellular Localization: Apical cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus. Membrane raft. Membrane; Single-pass type I membrane protein. Note=In single attached epithelial cells is restricted to a preapical pole on the free plasma membrane whereas other apical and basolateral proteins are not yet polarized Colocalizes with SLC9A3R2 at the apical plasma membrane during epithelial polarization. Colocalizes with SLC9A3R1 at the trans- Golgi network (transiently) and at the apical plasma membrane. Its association with the membrane raft is transient. Colocalizes with actin filaments, EZR and SLC9A3R1 in a punctate pattern at the apical cell surface where microvilli form. Colocalizes with EZR and SLC9A3R2 at the apical cell membrane of glomerular epithelium cells (By similarity). Forms granular, punctuated pattern, forming patches, preferentially adopting a polar distribution, located on the migrating poles of the cell or forming clusters along the terminal ends of filipodia establishing contact with the endothelial cells. Colocalizes with the submembrane actin of lamellipodia, particularly associated with ruffles. Colocalizes with vinculin at protrusions of cells. Colocalizes with ITGB1 Colocalizes with PARD3, PRKCI, EXOC5, OCLN, RAB11A and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis (By similarity).
宿主	Mouse
種屬反應(yīng)性	Human
免疫原	410-439 aa
靶點(diǎn)	This PODXL antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 410-439 amino acids from the C-terminal region of human PODXL.
克隆號(hào)	632CT5.11.4
亞型	IgG1
通用名	PCLP, PCLP1
基因ID	5420
UniProt ID	O00592
功能	Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
總結(jié)	This gene encodes a member of the sialomucin proteinfamily. The encoded protein was originally identified as animportant component of glomerular podocytes. Podocytes are highlydifferentiated epithelial cells with interdigitating foot processescovering the outer aspect of the glomerular basement membrane.Other biological activities of the encoded protein include: bindingin a membrane protein complex with Na+/H+ exchanger regulatoryfactor to intracellular cytoskeletal elements, playing a role inhematopoetic cell differentiation, and being expressed in vascularendothelium cells and binding to L-selectin.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	IF, WB, E
稀釋方法	IF~~1:100 WB~~1:500~16000
圖像	Fluorescent confocal image of SY5Y cells stained with PODXL (C-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AMM02439G PODXL (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). PODXL Antibody (C-term)(Ascites)(Cat. AMM02439G) western blot analysis in A549 cell line lysates (35μg/lane).This demonstrates the PODXL antibody detected the PODXL protein (arrow).
說(shuō)明書(shū)	Product Datasheet Protocol
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