ALPP Antibody (N-term)(Ascites)

AMM02400G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
AMM02400G		0.1 ml / ￥4950

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安諾倫（北京）生物科技有限公司

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ALPP Antibody (N-term)(Ascites)

品牌	Leading Biology	貨號	AMM02400G
產(chǎn)品分類	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ALPP Antibody (N-term)(Ascites).
分子量	57954 Da
細胞定位	Antigen Cellular Localization: Cell membrane; Lipid-anchor, GPI-anchor.
宿主	Mouse
種屬反應(yīng)性	Human
免疫原	56-83 aa
靶點	This ALPP antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 56-83 amino acids from the N-terminal region of human ALPP.
克隆號	421CT8.1.1
亞型	IgG1
通用名	PLAP
基因ID	250
UniProt ID	P05187
總結(jié)	There are at least four distinct but related alkalinephosphatases: intestinal, placental, placental-like, andliver/bone/kidney (tissue non-specific). The first three arelocated together on chromosome 2 while the tissue non-specific formis located on chromosome 1. The product of this gene is a membranebound glycosylated enzyme, also referred to as the heat stableform, that is expressed primarily in the placenta although it isclosely related to the intestinal form of the enzyme as well as tothe placental-like form. The coding sequence for this form ofalkaline phosphatase is unique in that the 3' untranslated regioncontains multiple copies of an Alu family repeat. In addition, thisgene is polymorphic and three common alleles (type 1, type 2 andtype 3) for this form of alkaline phosphatase have been wellcharacterized.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	IF, WB, E
稀釋方法	IF~~1:100 WB~~1:500~3200
圖像	Fluorescent confocal image of HeLa cells stained with ALPP (N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AMM02400G ALPP (N-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ALPP immunoreactivity is localized to the cytoplasm of HeLa cells. ALPP Antibody (N-term) (Cat. AMM02400G) western blot analysis in mouse placenta tissue lysates (35μg/lane).This demonstrates the ALPP antibody detected the ALPP protein (arrow).
說明書	Product Datasheet Protocol
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