品牌 |
Leading Biology |
貨號 |
AMM02378G |
產(chǎn)品分類 |
Monoclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ACTA1 Antibody.
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分子量 |
42051 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm, cytoskeleton.
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宿主 |
Mouse
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種屬反應(yīng)性 |
Human
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靶點(diǎn) |
This ACTA1 monoclonal antibody is generated from mouse immunized with ACTA1 recombinant protein.
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克隆號 |
337CT30.10.1
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亞型 |
IgG1,k
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通用名 |
ACTA
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基因ID |
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UniProt ID |
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功能 |
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
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總結(jié) |
The product encoded by this gene belongs to the actinfamily of proteins, which are highly conserved proteins that play arole in cell motility, structure and integrity. Alpha, beta andgamma actin isoforms have been identified, with alpha actins beinga major constituent of the contractile apparatus, while beta andgamma actins are involved in the regulation of cell motility. Thisactin is an alpha actin that is found in skeletal muscle. Mutationsin this gene cause nemaline myopathy type 3, congenital myopathywith excess of thin myofilaments, congenital myopathy with cores,and congenital myopathy with fiber-type disproportion, diseasesthat lead to muscle fiber defects.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:100~500
IHC-P~~1:10~50
FC~~1:10~50
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圖像 |
All lanes : Anti- at 1:1000 dilution
Lane 1: A431 whole cell lysate
Lane 2: Hela whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 42 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
ACTA1 Antibody(Cat. AMM02378G) western blot analysis in CEM cell line lysates (35μg/lane).This demonstrates the ACTA1 antibody detected the ACTA1 protein (arrow).
ACTA1 Antibody (Cat. AMM02378G)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjμgation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
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說明書 |
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數(shù)量 |
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