BMI1 Antibody

AMM02364G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
AMM02364G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

Tel: 400-965-8633
Tel: 010-56256916
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BMI1 Antibody

品牌	Leading Biology	貨號	AMM02364G
產(chǎn)品分類	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality BMI1 Antibody.
分子量	36949 Da
細胞定位	Antigen Cellular Localization: Nucleus. Cytoplasm.
宿主	Mouse
種屬反應(yīng)性	Human
靶點	This BMI1 monoclonal antibody is generated from mouse immunized with BMI1 recombinant protein.
克隆號	282CT3.7.6
亞型	IgG1,k
通用名	PCGF4, RNF51
基因ID	100532731;648
UniProt ID	P35226
功能	Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
總結(jié)	Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IF, E
稀釋方法	WB~~1:100~250 IF~~1:10~50
圖像	All lanes : Anti-BMI1 Antibody at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: A549 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST. BMI1 Antibody (Cat. AMM02364G) western blot analysis in K562 cell line lysates (35μg/lane).This demonstrates the BMI1 antibody detected the BMI1 protein (arrow). Confocal immunofluorescent analysis of BMI1 Antibody ( AMM02364G) with NCI-H460 cell followed by Alexa Fluor? 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
說明書	Product Datasheet Protocol
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