全部產(chǎn)品分類
品牌 |
Leading Biology | 貨號 |
LM21018 |
產(chǎn)品分類 |
單克隆抗體 | 研究領(lǐng)域 |
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產(chǎn)品概述 |
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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形式 |
The activation of protein tyrosine kinases is a critical event in T cell antigen receptor (TCR)-mediated signaling. One substrate of the TCR-activated protein tyrosine kinase pathway is a 76-kDa protein (SLP-76) that associates with the adaptor protein Grb2. Northern analysis demonstrates that pp76 mRNA is expressed solely in peripheral blood leukocytes, thymus, and spleen; and in human T cell, B cell and monocytic cell lines. In vitro translation of pp76 cDNA gives rise to a single product of 76 kDa that associates with a GST/Grb2 fusion protein, demonstrating a direct association between these two molecules. Results demonstrate that this novel protein is likely to play an important role in TCR-mediated intracellular signal transduction (1). It has been shown that both SLP-76 and ZAP70 co-immunoprecipitate with Shb in Jurkat T cells and that Shb and Vav co-immunoprecipitate when cotransfected in COS cells. Results indicate that upon TCR stimulation, Shb is targeted to these lipid rafts where Shb aids in recruiting the SLP-76-Gads-Vav complex to the T cell receptor zeta-chain and ZAP70 (2). Tyrosine phosphorylation of SLP-76 at position 113 or 128 was sufficient for it to associate with the N-terminal SH2 of p85. This data suggest that SLP-76 may play a role in PI3K signaling pathways (3).
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濃度 |
A phospho specific peptide corresponding to residues surrounding tyrosine 113 of human SLP-76. This antibody detects SLP-76 phosporylated on tyr113. |
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純度 |
Rabbit |
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試劑準(zhǔn)備 |
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
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復(fù)溶 |
1. Jackman JK, et al J Biol Chem 270(13):7029-32, 1995<br>2. Lindholm CK et al. Eur J Biochem 269(13):3279-88, 2002<br>3. Shim EK, et al. FEBS Lett 575(1-3):35-40, 2004<br>
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儲存條件 |
Human
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儲存溶液 |
Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein, SLP76, LCP2
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應(yīng)用 |
WB~~1:5000~10000
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產(chǎn)品描述 |
Function
Involved in T-cell antigen receptor mediated signaling.
Cellular_Location
Cytoplasm.
Tissue_Location
Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines Not detected in fibroblast or neuroblastoma cell lines
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數(shù)量 |
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