Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

The protein encoded by this gene is a member of the RASsuperfamily which are small GTP/GDP-binding proteins with anaverage size of 200 amino acids. The RAS-related proteins of theRAB/YPT family may play a role in the transport of proteins fromthe endoplasmic reticulum to the Golgi and the plasma membrane.This protein shares 97%, 96%, and 51% similarity with the dog RAB8,mouse MEL, and mouse YPT1 proteins, respectively and contains the 4GTP/GDP-binding sites that are present in all the RAS proteins. Theputative effector-binding site of this protein is similar to thatof the RAB/YPT proteins. However, this protein contains aC-terminal CAAX motif that is characteristic of many RASsuperfamily members but which is not found in YPT1 and the majorityof RAB proteins. Although this gene was isolated as a transforminggene from a melanoma cell line, no linkage between MEL andmalignant melanoma has been demonstrable. This oncogene is located800 kb distal to MY09B on chromosome 19p13.1.

This RAB8A monoclonal antibody is generated from mouse immunized with RAB8A recombinant protein.

Mouse

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Dong, C., et al. J. Biol. Chem. 285(26):20369-20380(2010) Knodler, A., et al. Proc. Natl. Acad. Sci. U.S.A. 107(14):6346-6351(2010) Davila, S., et al. Genes Immun. 11(3):232-238(2010) Linder, M.D., et al. Arterioscler. Thromb. Vasc. Biol. 29(6):883-888(2009) Xu, X.F., et al. Intervirology 52(5):258-265(2009)

Human, Mouse

Ras-related protein Rab-8A, Oncogene c-mel, RAB8A, MEL, RAB8

WB~~1:100

Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis. Cellular_Location Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus Recycling endosome membrane. Cell projection, cilium. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle, phagosome membrane; Lipid-anchor; Cytoplasmic side. Note=Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane. In the GDP- bound form, present in the perinuclear region. Shows a polarized distribution to distal regions of cell protrusions in the GTP- bound form. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis. Localizes to tubular recycling endosome. Recruited to phagosomes containing S.aureus or M.tuberculosis