Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Protein-kinase-C-related kinases (PRKs) (1) are part of the lipid-regulated protein kinases (PKC) which also include liver PAK & PKN (2-3). Human PRK1 and PRK2 share structurally similar catalytic domains, but less similar N-terminal regulatory regions suggesting different regulatory domain functions. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues. Additionally, the serine-threonine kinase PRK2 can be specifically cleaved by caspase-3 (and/or caspase-3-like subfamily members) during apoptosis (4).

A synthetic peptide corresponding to residues near N-terminus of human PRK2 was used as immunogen.

Rabbit

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

1. Nishizuka, Y (1992) Science 258, 607-614<br>2. Gabrielli, B., Wettenhall, R. E. H., Kemp, B. E., Quinn, M., and Bozinova, L. (1984) FEBS Lett. 175, 219-226<br>3. Mukai, H., and Ono, Y. (1994) Biochem. Biophys. Res. Commun. 199, 897-904<br>4. Palmer, R. H., and Parker, P. J. (1995) Eur. J. Biochem. 227, 344-351<br>

Human, Mouse, Rat

Serine/threonine-protein kinase N2, PKN gamma, Protein kinase C-like 2, Protein-kinase C-related kinase 2, PKN2, PRK2, PRKCL2

WB~~1:1000~5000 IHC~~1:250~500

Function PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c- fos serum response factor (SRF). Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication. Cellular_Location Cytoplasm. Nucleus. Membrane. Cell projection, lamellipodium. Cytoplasm, cytoskeleton. Cleavage furrow. Midbody. Cell junction. Note=Colocalizes with PTPN13 in lamellipodia-like structures, regions of large actin turnover Accumulates during telophase at the cleavage furrow and concentrates finally around the midbody in cytokinesis. Recruited to nascent cell-cell contacts at the apical surface of cells. In the course of viral infection, colocalizes with HCV NS5B at perinuclear region in the cytoplasm Tissue_Location Ubiquitous. Expressed in numerous tumor cell lines, especially in bladder tumor cells