Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Etk (epithelial and endothelial tyrosine kinase) is a member of the TEC family of non-receptor protein tyrosine kinase which includes Btk, Itk and TEC. Etk is composed of a pleckstrin domain, an SH3 and SH2 domain and a catalytic kinase domain (1). Etk is implicated in cell adhesion, migration, proliferation, survival as well as signal transduction (2). In PCA, Etk activation by IL-6 is mediated by PI3-K (3). It as also been shown in TNF-induced angiogenesis, TNF activates Etk through TNFR2 (4). Additionally, in the AR pathway, ETtk, FAK and Src form a complex. FAK activates Src which in turn activates Etk by phosphorylating Etk tyrosine residue 566 (5).

A synthetic peptide corresponding to residues in the pleckstrin homology domain of human ETK/BMX was used as immunogen.

Rabbit

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

1. Chen, R., O. Kim, M. Li, X. Xiong, J. L. Guan, H. J. Kung, H. Chen, Y. Shimizu, and Y. Qiu. 2001. Nat. Cell Biol. 3:439-444<br>2. Qiu, Y., and H. J. Kung. 2000. Oncogene 19:5651-5661<br>3. Yun Qiu, Dan Robinson, Tom G. Pretlow, and Hsing-Jien Kung, Cell Biology, Vol. 95, Issue 7, 3644-3649, March 31, 1998<br>4. Shi Pan, Ping An, Rong Zhang, Xiangrong He, Guoyong Yin, and Wang Min, Molecular and Cellular Biology, November 2002, p. 7512-7523, Vol. 22, No. 21.<br>5. Lee LF, Guan J, Qiu Y, Kung HJ. Mol Cell Biol. 2001 December; 21(24): 8385<br>

Human, Mouse, Rat

Cytoplasmic tyrosine-protein kinase BMX, Bone marrow tyrosine kinase gene in chromosome X protein, Epithelial and endothelial tyrosine kinase, ETK, NTK38, BMX

WB~~1:500~1000

Function Non-receptor tyrosine kinase that plays central but diverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility. Plays a critical role in TNF-induced angiogenesis, and implicated in the signaling of TEK and FLT1 receptors, 2 important receptor families essential for angiogenesis. Required for the phosphorylation and activation of STAT3, a transcription factor involved in cell differentiation. Also involved in interleukin-6 (IL6) induced differentiation. Plays also a role in programming adaptive cytoprotection against extracellular stress in different cell systems, salivary epithelial cells, brain endothelial cells, and dermal fibroblasts. May be involved in regulation of endocytosis through its interaction with an endosomal protein RUFY1. May also play a role in the growth and differentiation of hematopoietic cells; as well as in signal transduction in endocardial and arterial endothelial cells. Cellular_Location Cytoplasm. Note=Localizes to the edges of spreading cells when complexed with BCAR1 Tissue_Location Highly expressed in cells with great migratory potential, including endothelial cells and metastatic carcinoma cell lines