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促銷價(jià)：3500

品牌： ATCC細(xì)胞

貨號(hào)：     NK-92

生長(zhǎng)狀態(tài)：     懸浮生長(zhǎng)

運(yùn)輸方式：     T25瓶或干冰

器官來(lái)源：     血液系統(tǒng)

是否是腫瘤細(xì)胞：         是

細(xì)胞形態(tài)：     聚團(tuán)生長(zhǎng)

物種來(lái)源：     人源

組織來(lái)源：     血液

Novagen Catlog: NGT-CRL-2407

Cell name: NK-92

ATCC Number: CRL-2407

Depositors: Conkwest Inc.

Biosafety Level: 2

Growth property: Suspension, multicell aggregates

Morphology: Lymphoblast

Species: Homo sapiens (human)

Source: Cell type: natural killer cell; NK cell

Disease: malignant non-Hodgkin's lymphoma

Antigen Expressed: CD2 +, CD7 +, CD11a +, CD28 + , CD45 +, CD54 +, CD56 +, CD1 -, CD3 -,

CD4 -, CD5 -, CD8 -, CD10 -, CD14 -, CD16 -, CD19 -, CD20 -, CD23 -, CD34 -,

HLA-DR -

Ethnic origin: Caucasian

Age: 50 years

Gender: Male

Growth medium: Alpha Minimum Essential medium without ribonucleosides and deoxyribo-nucleosides

but with 2 mM L-glutamine and 1.5 g/L sodium bicarbonate + 0.2 mM inositol + 0.1 mM 2-mercaptoethanol + 0.02 mM folic acid + 100-200 U/ml recombinant IL-2 + 12.5% horse serum + 12.5% fetal bovine serum

Temperature: 37.0℃

Subculturing: Subcultivation Ratio: 1:2 to 1:3 is recommended Subculture every 2 to 3 days

Comments: NK-92 is an interleukin-2 (IL-2) dependent Natural Killer Cell line derived from peripheral

blood mononuclear cells from a 50 year old Caucasian male with rapidly progressive non-Hodgkin's lymphoma.

The cell line is dependent on the presence of recombinant Il-2 and a dose as low as 10 U/ml is sufficient to maintain proliferation; cells will die within 72 hours in the absence of IL-2.

The cell line is cytotoxic to a wide range of malignant cells; it kills both K562 cells and Daudi cells in chromium release assays.

NK-92 cells (after irradiation to prevent proliferation) can be used effectively for immunological ex vivo purging of leukemia from blood without compromising hematopoietic cell function.

NK-92 cells have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34 and HLA-DR.

Species determination: STR analysis (intraspecies) Human (Unique DNA Profile)

TH01: 6, 9.3

D5S818: 12 13

D13S317: 9, 12

D7S820: 10, 11

D16S539: 11, 12

CSF1PO: 11, 12

Amelogenin: X, Y

vWA: 18

TPOX: 8

1 March 2016 | Volume 7 | Article 91

Review

published: 14 March 2016

doi: 10.3389/fimmu.2016.00091

Frontiers in Immunology | www.frontiersin.org

1 November 2015 | Volume 6 | Article 578

Review

published: 17 November 2015

doi: 10.3389/fimmu.2015.00578

Frontiers in Immunology | www.frontiersin.org

該細(xì)胞在各大雜志上有報(bào)道，并且美國(guó)食品藥品監(jiān)督管理局（FDA）和倫理委員會(huì)（IRB）已經(jīng)批準(zhǔn)使用指定細(xì)胞庫(kù)提供的無(wú)病原體NK-92細(xì)胞，在德國(guó)進(jìn)行的臨床試驗(yàn)，且報(bào)道臨床二期的數(shù)據(jù)較為理想。

本株NK-92細(xì)胞由我公司安諾倫生物于16年2月引進(jìn)，傳代清晰，已做STR基因分型鑒定報(bào)告，并為該株細(xì)胞配套專用NK92無(wú)血清培養(yǎng)體系，使得這株細(xì)胞培養(yǎng)更為容易。

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